Table 2 Classification of histological score
Characteristics | Staining | Classification | ||
|---|---|---|---|---|
1 | 2 | 3 | ||
Disarray | H&E | Highly organized | Partially organized | Highly unorganized |
Angiogenesis | α-SMA | Sporadic blood vessels | Several blood vessels | Numerous blood vessels in whole tissue |
Fatty metaplasia | H&E | Absent | Focal | Extended |
Hypertrophy of synovium | H&E | 1 layer | 2–3 layers | 4–5 layers |
Immune cells | CD45 | Absent | Few immune cells mainly perivascular | Numerous immune cells inside the tissue |
Accumulation of fibroblasts | H&E | Only fibrocytes (small, elongated nucleus) | Slightly increased number of fibroblasts | Greatly increased number of fibroblasts |
Occurrence of villi | H&E | Absent | Focal | Extended |
Chondral metaplasia | Alcian Blue | Absent | Focal | Extended |
Nerve fibers | S100 | Absent | Few nerve fibers | Several nerve fibers |