Fig. 2
From: Effects of aging on the histology and biochemistry of rat tendon healing
Biochemical analysis of tenogenic and repair-related markers in patellar tendons from young and aged rats. (A). Quantitative reverse-transcription polymerase chain reaction was used to determine the gene expression levels of Col1a, TNMD, Scx, COMP, FMOD, MMP3 and TIMP3 in young and aged rat tendons at weeks 2 and 4. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. (B). Western blot analysis of the protein expression levels of Col-1 and MMP-3. GAPDH was used as an internal control. (C). Quantitative analysis of western blot results. *p < 0.05, **p < 0.01, ***p < 0.001, and n = 3. (D). Comparison of the expression levels of Col-1, Col-3, MMP-1 and TIMP-1 in young and aged rat tendons by immunohistochemical staining. Black scale bar: 20 um. (E). Quantitative analysis of the immunohistochemical staining of Col-1, Col-3, MMP-1 and TIMP-1. *p < 0.05, **p < 0.01, ***p < 0.001, and n = 5