Fig. 3
From: Dnmt3b ablation affects fracture repair process by regulating apoptosis
Ablation of Dnmt3b in led to delayed bone callus remodeling and decreased biomechanical properties of the newly formed bone during fracture repair. A Expression levels of Mmp13 genes in fracture calluses. Scale bar = 100 μm and Scale bar = 25 μm; B Immunohistochemical staining and quantification of Mmp13 in fracture calluses; C Tartrate-resistant acid phosphatase (TRAP) staining of fracture callus sections of Dnmt3bGli1ER mice and control mice at the indicated times. Scale bar = 200 μm; D Histomorphometry analysis of osteoclast surface per bone surface (Oc. S/BS) was performed on TRAP staining of Dnmt3bGli1ER and control fractures at the indicated times. E Maximum bending loading of fractured tibia 14 and 21dpf. F Maximum bending displacement of the fracture site 14 and 21dpf; Data were presented as means± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; n ≥ 4 in each group