Fig. 4

Dnmt3b deletion leads to the apoptosis of chondrocyte cells in the process of cartilage differentiation. A Immunohistochemical staining and quantification of Dnmt3b in fracture calluses Scale bar = 100 μm and Scale bar =25 μm; B TUNEL staining of chondrocytes in fracture calluses at 7dpf. Scale bar = 200 μm and Scale bar = 100 μm. C Immunohistochemical staining and quantification of anti-apoptotic factors, BCL-2, in fracture calluses. Scale bar = 200 μm and Scale bar = 50 μm. D Immunohistochemical staining and quantification of Caspase-3 in fracture calluses. Scale bar = 200 μm and Scale bar = 50 μm. E Immunohistochemical staining and quantification of apoptosis factors Caspase-8 in fracture calluses. Scale bar = 200 μm and Scale bar = 50 μm. Data were presented as means± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; n ≥ 3 in each group