Role of Galectin-3 in intervertebral disc degeneration: an experimental study

Table 1 RT-PCR primers

Gene	Primer	Sequence (5’-3’)	PCR Products
Rat GAPDH	Forward	ACAGCAACAGGGTGGTGGAC	253 bp
Rat GAPDH	Reverse	TTTGAGGGTGCAGCGAACTT	253 bp
Rat Mmp3	Forward	GAATCCCCTGATGTCCTCGT	150 bp
Rat Mmp3	Reverse	TTTTCGCCAAAAGTGCCTGT	150 bp
Rat aggrecan	Forward	TGGACTTGTCTCAGGTTTC	295 bp
Rat aggrecan	Reverse	AGTTGGGGCAGTTATGGAT	295 bp
Rat CCL3	Forward	GCATTTAGTTCCAGCTCAGTGA	161 bp
Rat CCL3	Reverse	GGACGGCAAATTCCACGAAA	161 bp
Homo GAPDH	Forward	TCAAGAAGGTGGTGAAGCAGG	115 bp
Homo GAPDH	Reverse	TCAAAGGTGGAGGAGTGGGT	115 bp
Homo aggrecan	Forward	TGAGCGGCAGCACTTTGAC	287 bp
Homo aggrecan	Reverse	TGAGTACAGGAGGCTTGAGG	287 bp
Homo CCL3	Forward	CATCACTTGCTGCTGACACG	192 bp
Homo CCL3	Reverse	CGCTGACATATTTCTGGACCC	192 bp
Homo Galectin-3	Forward	CCCTGTGGATGCTTCAAACC	162 bp
Homo Galectin-3	Reverse	GTAGGCCCCAGGATAGGAAG	162 bp
Homo Col II	Forward	TCCAGATGACCTTCCTACGC	209 bp
Homo Col II	Reverse	GGTATGTTTCGTGCAGCCAT	209 bp

ISSN: 1471-2474